Catharanthus roseus的問題，透過圖書和論文來找解法和答案更準確安心。 我們找到下列懶人包和總整理

Plant Biotechnology and Medicinal Plants: Periwinkle, Milk Thistle and Foxglove

為了解決Catharanthus roseus的問題，作者Rady, Mohamed Ramadan 這樣論述：

Plant tissue culture and advanced biotechnologies have proven to be influential tools that complement conventional breeding and accelerate development of many medicinal plants. Various approaches, such as pathway engineering, precursor feeding, transformation, elicitation with biotic and abiotic

elicitors and scaling up in bioreactors, have been explored to improve the production of secondary metabolites from different medicinal plants. This book provides a comprehensive description of various studies, carried out on in vitro culture and hairy root cultures of Catharanthus roseus, Silybum m

arianum and Digitalis species which have been considered as alternative sources for the production of anti-tumour compounds, flavonolignans and cardenolides. Specific focus is on elicitation strategy for increasing production of bioactive compounds of C. roseus L., S. marianum and Digitalis species

to overcome the constrains of conventional propagation. This book is valuable for researchers or students working on medicinal plants, phytochemistry, and plant tissue culture. It also serves as a reference for the pharmaceutical industry.

Catharanthus roseus進入發燒排行的影片

積雪草萃取物抑制登革病毒感染之體外評估

為了解決Catharanthus roseus的問題，作者鄭雅芝 這樣論述：

登革熱是一種由蚊子傳播的傳染病，流行於熱帶及亞熱帶地區。每年有超過 25 億人面臨感染風險，約有超過一億例病例，且有近 22,000 人死亡。在台灣，過去五年內發生登革熱病例超過1,600件。登革病毒是一種黃熱病毒，由3個結構蛋白和7個非結構蛋白組成，為單股正鏈RNA病毒。共有四種 DENV血清型。DENV通過雌性埃及斑蚊及白線斑蚊的叮咬感染人類，造成輕度至重度的臨床表現。積雪草(Centella asiatica)為繖形科植物，廣泛用作醫藥、化妝品、食品和飲料。積雪草含有多種活性化合物，如三萜類、聚乙炔類、黃酮類和甾醇類。積雪草對幾種病毒株具有抗病毒活性，例如人類免疫缺陷病毒、B型肝炎病毒

和單純皰疹病毒。然而，其抗登革病毒活性需要進一步研究。在本研究中，積雪草以18公升95%乙醇熱回流提取2小時，重複3次。積雪草乙醇萃取物被分配到正己烷、二氯甲烷、正丁醇和水層中。CCK-8測定結果表明CA-D在Vero細胞中具有低細胞毒性(CC50>100 μg/mL)。將積雪草二氯甲烷層萃取物(CA-D)與DENV-2感染Vero細胞2天，並通過免疫螢光染色、RT-qPCR、TCID50和西方墨點法，顯示CA-D的抗DENV-2活性(IC50 =22.03 μg/mL)。在Time-of-addition試驗中，在感染的同時給予萃取物，具有顯著的抑制活性，因此可以認為CA-D作用於DENV-

2感染的早期。此外本研究也發現，CA-D具有顯著的殺死病毒的活性。 CA-D經矽膠柱層析分離得到六層(CA-D1~D6)，CA-D5經葡萄糖凝膠LH-20管柱層析法及高效液相層析儀等方式，純化出三個主要化合物。CA-D2~D5表現出不同的抗DENV-2活性，但其主要三個化合物(madecassic acid、terminolic acid和asiatic acid)對DENV-2無明顯抑制作用。綜合以上結果顯示，CA-D對DENV-2感染具抑制作用，可能其他化合物具有發展為抗DENV之抗病毒藥物的潛力。

Plant Biotechnology and Medicinal Plants: Periwinkle, Milk Thistle and Foxglove

為了解決Catharanthus roseus的問題，作者Rady, Mohamed Ramadan 這樣論述：

Plant tissue culture and advanced biotechnologies have proven to be influential tools that complement conventional breeding and accelerate development of many medicinal plants. Various approaches, such as pathway engineering, precursor feeding, transformation, elicitation with biotic and abiotic

elicitors and scaling up in bioreactors, have been explored to improve the production of secondary metabolites from different medicinal plants. This book provides a comprehensive description of various studies, carried out on in vitro culture and hairy root cultures of Catharanthus roseus, Silybum m

arianum and Digitalis species which have been considered as alternative sources for the production of anti-tumour compounds, flavonolignans and cardenolides. Specific focus is on elicitation strategy for increasing production of bioactive compounds of C. roseus L., S. marianum and Digitalis species

to overcome the constrains of conventional propagation. This book is valuable for researchers or students working on medicinal plants, phytochemistry, and plant tissue culture. It also serves as a reference for the pharmaceutical industry.

兔眼藍莓葉之酚酸和類黃酮奈米乳化液製備和抗老化活性測定

為了解決Catharanthus roseus的問題，作者于馨絨 這樣論述：

兔眼藍莓 (Vaccinium virgatum) 屬杜鵑花科的常綠灌木，具有美味和高營養價值。藍莓葉富含酚酸與類黃酮，因此具有重要生理功效如抗氧化和抗癌活性，可被開發為保健食品。藍莓葉通常被當成廢棄物丟棄，但葉子的總多酚含量為果實三倍。本報告目的係由兔眼藍莓葉萃取酚酸和類黃酮，利用高效液相串聯質譜儀定性定量，製備奈米乳化液並測定抗小鼠老化活性。結果顯示，30% 乙醇為最適萃取酚酸和類黃酮溶劑，二十種不同兔眼藍莓葉品種中，NTU 104 品種具有較高含量之總酚酸 (136.09 mg/g)，Misty 品種則具有較高含量之總類黃酮 (111.36 mg/g)。使用 Waters Acquit

y UPLC BEH C18 管柱 (100 × 2.1 mm I.D., 1.7 μm particle size)，搭配高效液相三重四極桿式串聯質譜儀及電灑游離法, 在負離子模式下，使用選擇性反應監測模式進行分析，以 0.1% 甲酸水溶液 (A) 和 100% 氰甲烷 (B) 為梯度動相，流速 0.3 mL/min，管柱溫度 30℃，可於 7 分鐘內分離出 8 種酚酸與類黃酮。根據標準品之滯留時間和質荷比，可鑑定出藍莓葉含 5-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, quercetin-3-O-rutinoside, querceti

n 3-O-galactoside, quercetin 3-O-glucoside, kaempferol 3-O-glucoside, 3,5-dicaffeoylquinic acid 和4,5-dicaffeoylquinic acid。將萃取物與大豆油、Tween 80、甘油和去離子水按適當比例混合，搭配超音波均質機，可製備出平均粒徑為16 nm，界面電位為 -54 mV 的酚酸和類黃酮奈米乳液，經動態光散射儀、界面電位儀和穿透式電子顯微鏡測定可得知粒徑分佈、分散指標、界面電位和形狀，經 90 天儲藏與 100℃ 加熱後之粒徑分佈與界面電位顯示有良好安定性。動物實驗結果顯示，酚酸與類

黃酮萃取液及奈米乳化液皆可増加小鼠肝組織中超氧化物歧化酶 (superoxide dismutase)、麩胱甘肽過氧化物酶 (glutathione peroxidase) 及過氧化氫酶 (catalase) 活性，及増加小鼠腦組織中多巴胺 (dopamine) 含量，亦可降低小鼠腦組織中丙二醛 (malondialdehyde) 及蛋白質羰基 (protein carbonyl) 含量，其中又以高劑量奈米乳化液組效果較好，顯示其具有降低氧化壓力及延緩衰老之效果。綜合上述結果，高劑量之酚酸與類黃酮奈米乳化液具有開發成健康食品的潛力。