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Selling Boldly: Applying the New Science of Positive Psychology to Dramatically Increase Your Confidence, Happiness, and Sales

為了解決Revert back to you的問題，作者Goldfayn, Alex 這樣論述：

WALL STREET JOURNAL BESTSELLER IF YOU'RE IN SALES, FEAR HAS COST YOU MILLIONS OF DOLLARS, AND THIS BOOK IS FOR YOU.Fear is the reason most salespeople don't like to pick up the phone (salespeople average just four hours per week on the phone, and our job is to talk to humans ).Fear is the reason we

don't ask for the business more, even though our customers want to buy from us.Fear is the reason we don't offer our customers additional products and services, even though they would love to buy more from us.This book deals with that fear.You will learn exactly how to overcome this destructive fear

in sales, and replace it with confidence, optimism, gratitude, joy, and proactive sales work. These are the powerful principles in the new field of positive psychology which are transforming how we work and succeed. Selling Boldly is the first book that leverages positive psychology to help you sel

l more.You'll also learn a series of fast, simple sales-growth techniques--like how to add on to existing orders; and how to close 20% more quotes and proposals instantly; and how to properly ask for and receive referrals--that will grow your sales...dramatically and quickly.Alex Goldfayn's clients

grow their sales by 10-20% annually, every year, as long as they apply his simple approaches.YOU ALREADY KNOW WHAT TO DOI am not going to teach you much in this book that you don't already know.You're a professional salesperson. You do this for a living. You know, for example, that testimonials and

referrals are among the best ways we have to grow sales, right? But do you ask for them enough? Most people don't.You know that calling a customer on the phone is more effective than emailing her, but you still often revert to email.You know your customers buy other products and services that you ca

n help them with, but you don't ask them about these products. You'd like to help them, and they would like more of your help -- that is why they've been with you for five or ten or twenty years -- but nevertheless we don't ask them.There is a difference between knowing what to do, and actually doin

g it.I know you know.With Selling Boldly, we start to do what we already know. We will cover what keeps us from doing these things (fear), how to overcome it (by listening to your happy customers), and how to implement these simple but powerful sales growth techniques (by briefly planning them, also

doing them).Because sales growth comes from doing, not knowing.Today, we start doing.And growing.These approaches are laid out in this book, in precise detail, for you to implement in your own work.Alex doesn't hold anything back in this manual for selling more.What's the secret to selling more?The

re is no secret.There is no magic bullet.There is only the work.There are only the mindsets, and the communications.In Selling Boldly, Alex teaches readers how to attain these mindsets, and how to implement these communications, so that sales have no choice but to grow ALEX GOLDFAYN is the CEO of

The Revenue Growth Consultancy, a seven-figure solo con­sulting practice which adds 10% to 20% annual sales growth to clients by system­atically implementing the mindsets and actions in this book, with layers of accountability, recognition and reward. Alex does more than 50 speeches and workshops e

ach year, and regularly keynotes large annual association meetings as well as sales kickoff events.

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酵素處理米蛋白水解物之製備條件及功效性評估

為了解決Revert back to you的問題，作者陳慧如 這樣論述：

近年來，在保健食品的開發中，已經進行了許多來自於植物蛋白質的生物活性胜肽的使用與研究。具有生物活性的胜肽也已被當作藥物使用，用於治療目的，例如：調節糖尿病、調節膽固醇及調節血壓等慢性疾病。本研究擬以不同品種米為原料，探討不同品種米中米蛋白之性質，並以不同澱粉酶(細菌型及黴菌型)去除澱粉，建立適合的蛋白質水解模式，期待較低溫度的澱粉水解可以保留胜肽更多的生物活性及降低生產耗能。其次，以分子量劃分水解物，了解胜肽之分子量及胺基酸組成，作為往後應用及開發活性胜肽商品之參考。在抗氧化方面的結果顯示，細菌型澱粉酶處理的蛋白質水解物之總酚與類黃酮含量為1.23 ± 0.17 mg GA/g RPH及32

.2 ± 0.08 µg QE/g RPH；黴菌型澱粉酶處理的蛋白質水解物則為2.50 ± 0.76 mg GA/g RPH及26.0 ± 0.65µg QE/g RPH。ABTS自由基的半清除活性能力(IC50)為2940.7 ± 583.9 μg/mL；黴菌型澱粉酶處理的蛋白質水解物則為2076.0 ± 845.0 μg/m。DPPH自由基的半清除活性能力(IC50)為35.50 ± 1.8 mg/mL；黴菌型澱粉酶處理的蛋白質水解物則為42.58 ± 2.1 mg/mL。細菌型澱粉酶處理的蛋白質水解物的還原能力6.21 ± 1.24 mg VCE /g;黴菌型澱粉酶處理的蛋白質水解物的還

原能力6.95 ± 1.40 mg VCE /g。氧自由基吸收能力表現方面，細菌型澱粉酶處理的蛋白質水解物為31.25 µmol TE/g RPH；黴菌型澱粉酶處理的蛋白質水解則為473 µmol TE/g RPH。在皮膚保健能力中，抑制酪胺酸酶測試之結果顯示，細菌型澱粉酶處理的蛋白質水解物的IC 50 = 104.72 mg/mL;黴菌型澱粉酶處理的蛋白質水解物的IC 50 = 107.6 mg/mL。抑制玻尿酸酶測試之結果顯示，細菌型澱粉酶處理的蛋白質水解物的IC 50 = 7.61 mg/mL;黴菌型澱粉酶處理的蛋白質水解物的IC 50 = 8.91 mg/mL。黴菌型澱粉酶處理的蛋白質

水解物在皮膚保健相關能力表現上略差於細菌型澱粉酶處理的蛋白水解物，但抗氧化能力部份整體比細菌型澱粉酶處理的蛋白水解物佳，因此，後續實驗將選由黴菌型澱粉酶處理的蛋白質水解物進行以下的試驗。使用分子量切割(MWCO)為10及1 kDa 之薄膜將粗水解液做劃分，並輔以SEC-HPLC測定其分子量，酵素水解過程中，蛋白質的水解會產生不同分子量的胜肽，結果發現黴菌型澱粉酶處理的蛋白質水解物的分子量落在0.15 – 1081.77 kDa的範圍內。黴菌型澱粉酶處理的蛋白質水解物主要的分子量則為0.95、1.96及25.9 kDa的分子量分佈。結果顯示，利用Alcalase水解黴菌型澱粉酶處理的米蛋白粗萃物

，可以將蛋白質水解成分子量幾乎小於5 kDa的胜肽。不同區分物都含有較高含量的穀胺酸、天冬胺酸、精胺酸、白胺酸、酪胺酸、苯丙胺酸、丙胺酸及絲胺酸，其中，疏水性胺基酸((hydrophobic amino acids, HA)及芳香族胺基酸((aromatic amino acids, AAA)以MW < 1 kDa的含量最高，分別為272.6及98.6 mg/g。此外，支鏈胺基酸(branched chain amino acids, BCAA)的含量也是3種區分物中最高的(158.53 mg/g)。抗氧化能力部分，DPPH自由基清除能力的IC 50值範圍在26.7 - 39.4mg/mL之，

其中MW 10 kDa) 級分(IC50 值為 39.4 ± 1.19 mg/mL)顯著高於(p < 0.05)。清除ABTS自由基的IC50值。結果顯示 MW > 10 kDa的組分(IC50 = 1.29 ± 0.20mg/mL)在三個組分中是最有效的(p < 0.05)。結果顯示出，分子量較高的區分物(MW > 10 kDa)較分子量較高的區分物(MW < 1 kDa)具有更好的清除ABTS 自由基的能力。還原能力部分在MW < 1 kDa 區分中發現最高的鐵還原能力(1.6 ± 0.19 mg AAE/g水解物)，顯著高於 1-10 kDa 區分和MW > 10 kDa區分(p <

0.05)。另外，ORAC分析中的動態螢光衰減曲線，利用積分的方式計算曲線下的面積，經過計算得知MW < 1 kDa、MW 1 - 10 kDa和MW > 10 kDa，ORAC值分別為774、576和603 µmol TE/g RPH。利用皮爾森(Pearson)相關分析用於探討抗氧化能力與組成分之間的線性相關。由Pearson相關係數測試顯示ORAC與TPC顯著正相關(r = 0.999, p < 0.05)。DPPH和ABTS自由基清除活性的IC50與胺基酸組成分的含量呈現負相關(r = -0.986至-1.000)。胜肽中的胺基酸種類是決定生物活性的關鍵因素。芳香族胺基酸與還原能力顯著

相關(r = 0.997, p < 0.05)。另外，使用的黴菌型澱粉酶處理的蛋白質水解物MW< 1 kDa 區分物都減少了 LPS 刺激的炎症介質的產生。特別是，在劑量 200 μg/mL下顯示出對細胞激素的抑制效果最為顯著抑制(p < 0.05)。因此，可以得知黴菌型澱粉酶處理的蛋白質水解物MW< 1 kDa 區分物可能對 LPS 誘導的炎症最有效的治療作用。本研究開發至今，嘗試不同的米的品種及澱粉去除方式獲得米蛋白粗萃物，並且水解之後探討水解的最大效應，進行對皮膚保健及抗氧化能力的篩選，選定以黴菌型澱粉酶處理的蛋白質水解物進行不同分子量的區分，也提出許多的體外試驗數據，蛋白質來源也選擇無

過敏源問題的米蛋白做為主題，探討其對抗氧化能力及初步的抗發炎能力。

Intermittent Fasting: The Fast Diet Plan to Weight Loss Success & Longevity

為了解決Revert back to you的問題，作者Greene, Stephany J. 這樣論述：

Shifting Your Eating Pattern Is So Much More Effective Than Dieting Intermittent Fasting: A Weight Loss Revolution Are you just as sick of the next 'revolutionary diet' as I am? Whether you tried to do a Ketogenic diet, Mediterranean diet, or one of the million Green Smoothie recipes... Nothing seem

s to really work out. Why? Because they demand you to change the yummy things you like to eat. What if we could simply change the pattern of eating, instead of what we eat? This is not 'just another cookbook', but it's a helpful guide of knowledge to changing your eating pattern for weight loss. Th

e expert analysis by author Stephany J. Greene will help and motivate you to change your life for the better. What can I expect to read in this book?Exactly What Fasting Is & How It Helps Your Body Lose WeightThe 5 Key Methods To Intermittent Fasting (Tailored to Personal Needs)Useful Tips on How to

Start Your First Fasting PeriodThe Many Practical Benefits To Your Health & Personal LifestyleWhat Fasting Methods Are Suitable For Which Types of PeopleNegative Side-Effects of Intermittent Fasting (And How To Combat Them)The Best Practices to Follow When Practicing Fasting And much, much more Br

inging Your Body Back In A Natural State Fasting to lose weight seems impossible. But it's actually a second nature for the human body Ever since we were hunter-gatherers, humans have experienced periods of fasting. Simply because in ancient civilization, food was not always widely available. Socie

ty has changed now: we live in a world of abundance. Our body still hasn't adapted to this fact, making fasting a great opportunity to revert back to our 'ancient' eating pattern. The different methods of intermittent fasting are a life-changing opportunity. The best part? It's not even a diet Alo

ng the way you can eat whatever you prefer (with minor guidelines), it is only the moment of eating that changes with fasting. Start learning right away by clicking the ADD TO CART button at the top of this page, and enjoy reading immediately. Stephany J. Greene is an expert in dieting, with her

own weight loss clinic in California, USA. Her second passion is writing to help people with diet-related problems.

毛囊經輻射傷害後的再生反應：異位前驅細胞的角色

為了解決Revert back to you的問題，作者黃文彥 這樣論述：

器官在結構變換的過程中如何妥善佈署幹細胞，對於生理恆定及損傷後的再生皆扮演著關鍵的角色。毛囊生長週期包含三個時期，分別是生長期(anagen)、衰退期(catagen)、及休止期(telogen)，各時期的毛囊結構與細胞組成皆不盡相同。在此，我們利用毛囊當作模式來探討器官結構變換時，幹細胞的佈署與器官再生之間的關聯性。在臨床上利用放射線或化學治療癌症的病人都不可避免地伴隨著許多副作用，落髮是其中的一種，且目前並無有效的治療方法，造成病患除了生理的不適外也承受了心理上極大壓力。臨床上，生長期毛囊受損的反應及受損後毛囊再生的機轉還有待更深入瞭解。為了讓基礎研究的結果能解決臨床上的問題，我們使用臨

床上廣泛用來治療癌症的游離輻射與化學治療藥cyclophosphamide誘導生長期毛囊的損傷，並探討以下問題: (1)游離輻射與cyclophosphamide造成的細胞效應，(2)再生過程中不同細胞族群的動態，(3)毛囊萎縮與再生過程中的訊息調控機制。我們發現游離輻射和cyclophosphamide主要在基質細胞引起DNA雙股斷裂並造成細胞凋亡，其受損的程度隨劑量增加而遞增。另高劑量的游離輻射與cyclophosphamide皆造成與臨床上相似的落髮現象。結合BrdU標定與細胞系追蹤，我們發現根據受損程度的不同，生長期毛囊可透過異位前驅細胞的調動與去分化這兩種不同機制，使毛囊再生進而防止

繼續萎縮。在低劑量游離輻射後，毛囊球基底層的K5+細胞會被快速活化，並在12到72小時內補充毛球內因細胞凋亡而失去的細胞。我們稱此為早期再生反應。在早期再生反應中，突部毛囊幹細胞則維持靜止狀態並無參與此再生過程。當高劑量輻射造成所有的基質細胞與毛球基底層K5+細胞都走向細胞凋亡時，這時存活下來的外根鞘細胞即透過去分化獲得與正常休止期毛囊中的次級毛胚幹細胞相似的幹細胞特性，並在72到120小時內進行毛囊再生。我們稱此為晚期再生反應。突部毛囊幹細胞則在輻射後第五天活化並分化成上部的外根鞘細胞。免疫染色與次世代定序分析結果顯示，游離輻射傷害會誘導p53表現，並且短暫抑制Wnt signaling。7

2小時之後，當p53停止表現，Wnt signaling即再度被活化。若在毛囊啟動自發性再生反應之前給予WNT 抑制劑或是剔除Wnt下游基因表現，毛囊就無法再生。此結果顯示毛囊再生反應的啟動與否需要Wnt signaling來調控。於是我們嘗試在高劑量游離輻射與cyclophosphamide傷害後立即以皮下植入方式給予Wnt3a重組蛋白，此處理雖無法降低p53表現，但透過Wnt3a持續活化Wnt signaling，進而刺激異位前驅細胞的活化，可以防止輻射及化療造成的落髮。我們的研究結果顯示生長期的毛囊透過廣泛佈署多能性細胞以因應損傷發生時，毛囊能迅速再生。透過活化異位前驅細胞可做為一個新的

策略來改善臨床上因放射線或化療造成的落髮。