Perpetuating factor的問題,透過圖書和論文來找解法和答案更準確安心。 我們找到下列懶人包和總整理

Perpetuating factor的問題,我們搜遍了碩博士論文和台灣出版的書籍,推薦Ruhiiga, Nathan寫的 Once upon a Farm: Introducing Sustainable Agriculture to Young Readers 和Delaune, Valerie的 Trigger Point Therapy for Headaches & Migraines: Your Self-Treatment Workbook for Pain Relief都 可以從中找到所需的評價。

這兩本書分別來自 和所出版 。

長庚大學 生物醫學研究所 郁兆蘭所指導 陳思元的 鑑別細胞週期蛋白依賴性激酶1(CDK1)為致癌性淋巴細胞特異性蛋白酪氨酸激酶(Lck)的潛在磷酸化標的物 (2021),提出Perpetuating factor關鍵因素是什麼,來自於淋巴細胞特異性蛋白酪氨酸激酶、細胞週期蛋白依賴性激酶1、粒線體、蛋白質體學、白血病、酪氨酸磷酸化。

而第二篇論文國立臺北教育大學 心理與諮商學系碩士班 蔡松純所指導 廖品筑的 職場無禮與員工身心健康:反芻反應雙概念模式之中介效果探討 (2021),提出因為有 反芻反應、深思反省、苦惱自責、職場無禮、員工身心健康的重點而找出了 Perpetuating factor的解答。

接下來讓我們看這些論文和書籍都說些什麼吧:

除了Perpetuating factor,大家也想知道這些:

Once upon a Farm: Introducing Sustainable Agriculture to Young Readers

為了解決Perpetuating factor的問題,作者Ruhiiga, Nathan 這樣論述:

Irresponsible exploitation of our natural resources which has led to severe environmental degradation, in turn leading to climate change, coupled with the agrarian revolution that has promoted the excessive use of earth-polluting agro-chemicals are some of the more obvious causes of this predicament

. It is now clear that food security and indeed environmental protection can only be achieved by promoting sustainable agricultural principles such as organic farming and integrated pest management. There are many indicators being looked at in current sustainable agricultural research, encompassing

a myriad of aspects, some ecological/environmental, some economic, some social and even governance issues. Agriculture is not self-perpetuating, but relies on the human aspect to survive, let alone evolve. A lot of studies are being conducted and considerable effort is being directed towards promoti

ng sustainable agriculture for future food security, but a critical aspect of this equation has thus far not received the attention it deserves. This key part of the equation is the succession factor. The sustainability question, be it in politics, business or in agriculture, is incomplete without a

ddressing succession. Succession guarantees continuity, thereby securing tomorrow. Modern day children are losing touch with nature in general and farming in particular. According to a recent study by the BBC, just 1 in 5 modern day children are connected to nature (BBC, 2013). The study defined the

term "connected to nature" as; having empathy for creatures; having a sense of oneness with nature; having a sense of responsibility for the environment; enjoyment of nature. Urban-raised children who spend their leisure time on cartoons and bouncing castles are even more clueless, many of whom thi

nking that the food served at their dining tables is manufactured in the supermarket Due to the post-colonial shift towards formal education and modernization of African society, love for and experience with agriculture has been dwindling. This dangerous trend needs to be curtailed if agriculture i

s to remain sustainable. If food security and environmental protection are to be realized in the future, today's young learners, who will be tomorrow's decision-takers and policy-makers and indeed nature's stewards must be induced and inspired to not only understand and appreciated farming and natur

e conservation, but to also love the amazing art and science of producing food. A reorientation is necessary for these youngsters to look beyond video games and smart phones; beyond amusement parks and shopping sprees; to view soil not as a dirty and messy affair but as an amazing source of plant li

fe. Although the education curriculum incorporates agricultural studies at some point in secondary school, this tends to come in too little too late, at a time when most students have already shaped a less favorable attitude towards farming/natural sciences. A more elementary approach is needed to i

ntroduce the idea of farming to primary school pupils. The heart of this project is to stimulate interest for nature in the developing minds of our children; to ignite a love for farming at a formative stage. An appreciation for the basic art and science of growing food will also instill a love for

healthy eating among the young generation, who are increasingly getting hooked on junk food. Thus far, greater effort has been geared towards trying to create change through intervention. This project idea aims to create change through education. Mind-set change is more probable when tackled at form

ative stages of human development. There is no better place to start than in the minds of our children, who are curious, eager to learn and not boxed in.

鑑別細胞週期蛋白依賴性激酶1(CDK1)為致癌性淋巴細胞特異性蛋白酪氨酸激酶(Lck)的潛在磷酸化標的物

為了解決Perpetuating factor的問題,作者陳思元 這樣論述:

目錄中文摘要………………………………………………………………i英文摘要…………………………………………………………………ii目錄……………………………………………………………………iii圖目錄…………………………………………………………………viii表目錄……………………………………………………………………x附錄目錄………………………………………………………………xi第一章 緒論……………………………………………………………11.1 蛋白質酪氨酸激酶 (Protein tyrosine kinases,PTKs)…………11.2 淋巴細胞特異性蛋白酪氨酸激酶 (Lymphocyte-spec

ificprotein tyrosine kinase,Lck)…………………………………31.3 PTKs在細胞內的運輸 (Intracellular trafficking)……………51.4 蛋白質體學 (Proteomics)………………………………………61.5 細胞週期蛋白依賴性激酶1 (Cyclin-dependent kinase 1)………81.6 研究動機與目的…………………………………………………12第二章 研究材料與方法………………………………………………132.1 抗體 (Antibody)………………………………………………132.2 細胞株 (Cell Line)

……………………………………………142.3 建構穩定細胞株(Stable Cell Line)…………………………142.3.1 Lck基因………………………………………………………142.3.2 質體轉染 (Transfection)…………………………………152.3.2.1 磷酸鈣-DNA共沉澱法 (Calcium phosphate-DNAcoprecipitation)………………………………………152.3.2.2 Neon電穿孔轉染系統 (Neon ElectroporationSystem)………………………………………………162.3.3 篩選穩定表達外源基因細胞株…………

…………………172.4 細胞培養 (Cell culture)……………………………………172.5 細胞分餾 (Cell fractionation)……………………………182.6 萃取蛋白質 (Protein extraction)…………………………202.6.1 萃取細胞蛋白質……………………………………………202.6.2 萃取粒線體蛋白質…………………………………………212.7 蛋白質定量 (Protein quantification)……………………212.8 SDS聚丙烯醯胺凝膠電泳 (SDS-PAGE)……………………222.8.1 配置膠體…………………………………

…………………222.8.2 配置蛋白質樣品……………………………………………232.8.3 凝膠電泳……………………………………………………232.9 西方墨點法 (Western blot)…………………………………242.9.1 蛋白質轉印…………………………………………………242.9.2 免疫墨點法…………………………………………………252.10 抗體去除………………………………………………………252.11 磷酸蛋白質體學實驗 (Phosphoproteomics)………………262.11.1 溶液中蛋白質水解 ………………………………………262.11.2 脫鹽 …………………

……………………………………262.11.3 富集磷酸化胜肽片段 ……………………………………272.11.4 富集酪氨酸磷酸化胜肽片段 ……………………………292.11.4.1 4G10免疫沉澱…………………………………………292.11.4.2 鹼處理 …………………………………………………302.11.5 一維液相層析串聯式質譜分析(1D LC-MS/MS)………302.12 電子傳遞鏈複合物I活性實驗分析 (Electron transportchain complex I activity assay)………………………………312.13 細胞氧氣消耗分析(Oxygen consu

mption)…………………322.14 銀染實驗(Silver staining)……………………………………342.15 代謝體學實驗(Metabolomics experiment)…………………362.16 統計方法………………………………………………………36第三章 實驗結果………………………………………………………383.1 利用磷酸蛋白質體學尋找在白血病細胞粒線體中潛在的Lck標的蛋白質………………………………………………………383.1.1 建立專一性表達粒線體Lck的細胞株……………………383.1.1.1 利用限制酶切割確認可以專一性表達粒線體Lck的質體之正確性……………

…………………………………383.1.1.2 利用基因定序確認專一性表達粒線體Lck質體的Mitotarget序列之正確性…………………………………393.1.1.3 利用細胞分餾實驗及西方墨點法確認Lck是否專一性表達於粒線體中…………………………………………403.1.2 選定使用在磷酸蛋白質體學的白血病細胞……………413.1.2.1 鑑定小鼠白血病細胞LSTRA、EL4在粒線體中活化態Lck及酪氨酸磷酸化蛋白質的表達水平……………413.1.2.2 鑑定LSTRA粒線體功能下降與活化Lck的關係……423.1.3 對磷酸蛋白質體學中使用的樣品進行品質管控…………443.1.4 利用磷酸

蛋白質體學找尋在白血病細胞粒腺體內被Lck磷酸化的潛在標的蛋白質……………………………………443.2 鑑定Lck與CDK1的調控關係及對粒線體功能的影響……463.2.1 利用細胞分餾實驗及西方墨點法觀察在LSTRA及EL4細胞中Lck與CDK1的調控關係………………………………473.2.2 探討在EL4細胞中表達突變型Y505F Lck對CDK1………483.2.2.1 建立表達外源性Y505F Lck的EL4細胞……………483.2.2.2 利用細胞分餾實驗及西方墨點法觀察表達突變型Y505FLck的EL4細胞與正常表型EL4細胞中Lck與CDK1的調控關係……………………………………

……493.2.3 分析EL4/Vector及EL4/Y505F- Lck兩細胞之電子傳遞鏈複合物I 功能…………………………………………………503.2.4 對LSTRA、EL4/Vector及EL4/Y505F-Lck細胞進行氧氣消耗分析……………………………………………513.2.5 探討Lck活性受到抑制後CDK1的變化…………523.2.5.1 Lck抑制劑處理LSTRA細胞……………………………523.2.5.2 利用細胞分餾實驗及西方墨點法觀察經過Lck抑制劑處理的LSTRA細胞中Lck與CDK1的調控關係……53第四章 討論……………………………………………………………54第五

章 結論……………………………………………………………60參考文獻 ………………………………………………………………61圖表 ……………………………………………………………………73附圖……………………………………………………………………112圖目錄圖一、 確認質體之正確性……………………………………………73圖二、 利用基因定序確認專一性表達粒線體Lck質體中Mito target序列之正確性…………………………………………………75圖三、 確認將專一性表達粒線體蛋白質質體轉染的HEK293細胞,在各胞器部分表達Lck的情形………………………………76圖四、 確認白血病細株LSTRA、EL4

、Jurkat、JCam活化態Lck及Lck的表達……………………………………………………78圖五、 確認白血病細胞株在粒線體及細胞質酪氨酸磷酸化蛋白質表達差異……………………………………………………80圖六、 對EL4和LSTRA的細胞氧氣消耗分析……………………81圖七、 蛋白質體學樣品分析及品質管控……………………………82圖八、 磷酸化蛋白質體學實驗流程…………………………………84圖九、 以銀染實驗確認蛋白質樣品水解為胺基酸…………………85圖十、 兩項富集酪氨酸磷酸化胜肽片段實驗方法,使用4G10抗體免疫沉澱及使用NaOH鹼處理………………………………86圖十一、 對比在LSTRA

、EL4兩細胞中pCDK1 Y15磷酸化比例及CDK1在各胞器中表達量……………………………………88圖十二、 確認線性化質體DNA………………………………………91圖十三、 確認在EL4細胞使用Neon電穿孔轉染實驗條件………92圖十四、 確認Lck穩定轉染EL4細胞表達Lck蛋白質情形………93圖十五、 對比在EL4/Vector及EL4/Y505F-Lck兩細胞中pCDK1Y15磷酸化比例及CDK1在各胞器中表達量…………94圖十六、 分析EL4/Vector及EL4/Y505F-Lck 兩細胞之電子傳遞鏈Lck兩細胞之粒線體複合物I功能活性…………………97圖十七、 對LSTRA、EL

4/Vector及EL4/Y505F-Lck之細胞氧氣消耗分析……………………………………………………99圖十八、 確認使用不同濃度Lck抑制劑處理LSTRA細胞後抑制Lck在Y394磷酸化的結果……………………………………100圖十九、 進一步確認Lck抑制劑處理在LSTRA細胞中抑制Lck磷酸化的效果及專一性……………………………………102圖二十、 對比在LSTRA及經過Lck抑制劑處理的LSTRA兩細胞中pCDK1 Y15磷酸化比例及CDK1在各胞器中表達量…104圖二十一、 Lck通過CDK1導致粒線體功能下降的潛在機制……105表目錄表一、 LSTRA細胞粒線體萃取物利用蛋白質體學

在經過TiO2純化磷酸化胜肽片段再進行免疫沉澱後由4G10抗體抓取之酪氨酸磷酸化胜肽片段及所屬蛋白質身份……………………106表二、 LSTRA細胞粒線體萃取物利用蛋白質體學在經過TiO2純化磷酸化胜肽片段再進行免疫沉澱後未被4G10抗體抓取之酪氨酸磷酸化胜肽片段及所屬蛋白質身份…………………107表三、 LSTRA細胞粒線體萃取物利用蛋白質體學在經過TiO2純化磷酸化胜肽片段再進行NaOH鹼處理匯集的酪氨酸磷酸化胜肽片段及所屬蛋白質身份…………………………………109表四、 EL4細胞粒線體萃取物利用蛋白質體學在經過TiO2純化磷酸化胜肽片段再進行NaOH鹼處理匯集的酪氨酸磷酸化胜肽片段及所

屬蛋白質身份…………………………………………111 附圖目錄附圖一、 在Jurkat粒線體中Lck導致粒線體功能下降的潛在機制…………………………………………………………112附圖二、 利用代謝體學實驗分析LSTRA及EL4細胞中糖解作用(Glycolysis)及三羧酸循環(TCA cycle)的中間產物…114附圖三、 利用代謝體學實驗結果分析出LSTRA及EL4細胞產生ATP的比值……………………………………………………115附圖四、 細胞週期蛋白B1/CDK1與粒線體的調控………………116

Trigger Point Therapy for Headaches & Migraines: Your Self-Treatment Workbook for Pain Relief

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為了解決Perpetuating factor的問題,作者Delaune, Valerie 這樣論述:

I have such a bad headache For many of us, this is an oft repeated cry of anguish. Statistics about headaches and migraines are downright astonishing: One in six people have frequent headaches, and of those, one in ten suffers from migraines. You are not alone. Headaches and migraines result in mor

e than 10 million doctor visits each year. If you struggle frequently with headache pain, this book offers a welcome solution. Trigger point therapy is an effective self-care approach you can use to get relief from headache pain.Trigger points form in a portion of the muscle cell where blood flow is

reduced and metabolic wastes are not being exchanged for oxygen and nutrients. When enough trigger points are located together, they can form palpable knots in the tissue. Trigger points can cause pain, either in the area of the trigger point, or by referral to other areas of the body. Trigger poin

ts can be treated by applying pressure to affected areas, often providing instant relief. This book explains trigger point physiology and then offers a complete program for self-care that includes detailed illustrations of all pressure and stretching techniques. In addition, it provides an in-depth

look at the factors that cause and perpetuate trigger points, such as body mechanics, injuries, diet and nutritional deficiencies, hormonal imbalances, and emotional factors, and provides solutions to address each perpetuating factor.

職場無禮與員工身心健康:反芻反應雙概念模式之中介效果探討

為了解決Perpetuating factor的問題,作者廖品筑 這樣論述:

  反芻反應被證實與個人的身心狀態存在關聯性,並且反芻反應根據其內涵可以區分為兩種不同的類型:苦惱自責與深思反省。本研究企圖在職場脈絡下,探討不同類型的反芻反應,對於職場無禮與員工之身心健康間的中介效果與差異。  本研究採取立意抽樣法,以目前正在台灣組織中就業的成年人為研究對象,總共收集正式的有效問卷共658份。研究工具為「中文版反應風格量表修訂短版」、「知覺職場無禮量表改版」、「職業壓力指標測驗」中的「心理健康量表」與「生理健康量表」。  研究結果如下:一,職場無禮對員工的身心健康具有負向的預測效果;二,職場無禮對於反芻反應具有正向的預測效果;三,「苦惱自責」與「深思反省」都可以負向預測員

工的心理與生理健康;四,職場無禮會透過「反芻反應」的雙中介變項負向影響員工的心理健康與生理健康。在心理健康的中介效果上,「苦惱自責」比「深思反省」具有更強的負面中介效果;在生理健康的中介效果上,則是「深思反省」比「苦惱自責」具有更強的負面中介效果。透過本研究結果,增補了不同類型反芻反應的效果與作用差異,研究者亦根據研究結論提出在未來研究方向、組織實務與諮商實務上的建議。